This is from a very useful site, with troubleshooting tips for PCR. They have discussed each 'trouble' you can have with possible solutions, stepwise and in detail.
This is like the last option, the last 'resort' kind of thing, and its really funny!
The Bad Karma Hypothesis:
Background:
This is basically the "God is punishing you" hypothesis. It sometimes gains a great deal of favor.
Symptoms:
i) All rational explanations have been exhausted and yet PCR still is not working for you.
ii) Persistent feelings of guilt (if you are a Catholic, this symptom could be misleading).
Tests and solutions:
i) Try bungee jumping. If you survive, God must not be too hacked-off at you.
ii) Atone for your sins and start over at the top of the flow chart.
iii) If you end up here next time, have someone watch you next time you set up your PCR reactions.
I so identify with this!
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